Simultaneous Determination of Oleanic Acid and Ursolic Acid in Salvia Plebeia R. Br. from Different Ori-gins by HPLC
- VernacularTitle:HPLC法同时测定不同产地荔枝草中齐墩果酸和熊果酸含量
- Author:
Ming LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Salvia Plebeia R. Br.;
Oleanic acid;
Ursolic acid
- From:
China Pharmacist
2016;19(9):1776-1777,1780
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the simultaneous determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins. Methods:The column was Agilent-C18 (250 mm × 4. 6 mm,5μm) with the temperature of 25℃, the mobile phase was methanol-water-glacial acetic acid-triethylamine (90 ∶10 ∶0. 03∶0. 06) at the flow rate of 1. 0 ml·min-1 ,and the detection wavelength was 210 nm. Results: The calibration curves were linear within the range of 7. 50-75. 00 μg · ml-1 for oleanic acid(r=0. 999 7)and 5. 00-50. 00 μg · ml-1 for ursolic acid(r=0. 999 7). The average recovery of oleanic acid and ursolic acid was 100. 15%(RSD=1. 14%,n=6)and 99. 40%(RSD=1. 67%,n=6), respectively. Conclusion: The method is simple and accurate, which is suitable for the determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins.
