Determination of Nectandrin B in Uygur Medicine Arillus Myristicae by HPLC
- VernacularTitle:维吾尔药肉豆蔻衣中甘密脂素B的HPLC含量测定
- Author:
Xia YU
;
Shuyun WANG
;
Dahong YAO
;
Huijuan MAO
;
Jian HUANG
;
Guoyu LI
;
Jinhui WANG
;
Hangyu WANG
- Publication Type:Journal Article
- Keywords:
Arillus Myristicae;
Nectandrin B;
HPLC
- From:
China Pharmacist
2016;19(9):1756-1757,1758
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for determining nectandrin B in Uygur medicine Arillus Myristicae. Meth-ods:The analysis was performed on a SunFireTM C18 column (150 mm × 4. 6 mm, 5 μm) at 30°C using methanol /water (52 ∶48) as the mobile phase at a flow rate of 1. 0 ml·min-1 with the UV detection wavelength at 228. 4 nm. Results:The linear range of nectan-drin B was 3. 98 -79. 60 μg·ml-1(r=0. 999 2). The average recovery was 99. 89% with RSD of 1. 11%(n=6). Conclusion:The method is accurate, convenient and reproducible in the determination of nectandrin B in Uygur medicine Arillus Myristicae.
