Content Determination of Ethacridine Lactate in Compound Ethacridine Ointment by HPLC
10.6039/j.issn.1001-0408.2016.15.31
- VernacularTitle:HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量
- Author:
Junli LIU
;
Jiayi TIAN
;
Songleng DUAN
;
Weixin ZENG
;
Rui JIN
;
Lulu SUN
- Publication Type:Journal Article
- Keywords:
Compound ethacridine ointment;
Ethacridine lactate;
Content determination;
HPLC
- From:
China Pharmacy
2016;27(15):2109-2110,2111
- CountryChina
- Language:Chinese
-
Abstract:
OBJETCTIVE:To establish a method for the content determination of ethacridine lactate in Compound ethacridine ointment. METHODS:HPLC was performed on the column of Agilent ZORBAX SB-C18 with mobile phase of 0.1% Octanesulfon-ic acid sodium solution-acetonitrile(70∶30,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 270 nm,the column temperature was 30℃,and the injection volume was 10 μl. RESULTS:The linear range of ethacridine lactate was 10.002-50.010μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were less than 1%;recovery was 98.96%-100.36%(RSD=0.49%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for content determina-tion of ethacridine lactate in Compound ethacridine ointment.
