Determination of 100 Pesticide Residues in Moras alba by LC-MS/MS

VernacularTitle:LC-MS/MS法测定桑叶药材中100种农药残留量
Author: Lili HUANG ; Lili LI ; Yi LUO ; Minyi LU ; Qingquan HUANG
Publication Type:Journal Article
Keywords: Pesticide; Morus alba; LC-MS/MS
From: China Pharmacy 2016;27(15):2122-2125,2126
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the determination of 100 pesticide residues in Morus alba. METHODS:LC-MS/MS was conducted on the column of ZORBAX Eclipse plus C18 with mobile phase of acetonitrile (containing 5% water, 0.1% formic acid,5 mmol/L ammonium formate)- water(containing 0.1% formic acid,5 mmol/L ammonium formate)(gradient elution)at a flow rate of 0.4 ml/min,column temperature was 40 ℃,injection volume was 5 μl;MS conditions:ionization source was electrospray ionization with positive ion mode,scanning mode was dynamic MRM,scanning time window was 2 min;atomiz-ing gas was nitrogen,atomizing gas pressure was 40 psi,drying gas flow rate was 5 L/min,capillary voltage was 4 000 V,and ion spray voltage was 500 V. RESULTS:The linear range of 100 pesticide residues was 2.4-150 ng/ml(r>0.990 0),recovery was 69.3%-128.2%,and the determination limit was 0.003-16 μg/kg. CONCLUSIONS:The method is simple,stable and reproduc-ible,and can be used for the determination of pesticide residues in M. alba.