Simultaneous Determination of Three Constituents in Shenqi Xinshu Capsule by HPLC
10.6039/j.issn.1001-0408.2016.15.42
- VernacularTitle:HPLC法同时测定参七心疏胶囊中3种成分的含量
- Author:
Rongcheng YAO
;
Yuan DONG
;
Wenjie ZHANG
- Publication Type:Journal Article
- Keywords:
Shenqi xinshu capsule;
Notoginsenoside R1;
Ginsenoside Rg1;
Ginsenoside Rb1;
Content determination;
HPLC
- From:
China Pharmacy
2016;27(15):2141-2143,2144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of notoginsenoside R1,ginsenoside Rg1 and gin-senoside Rb1 in Shenqi xinshu capsule. METHODS:HPLC was performed on the column of Zorbax SB-C18(150 × 4.6 mm,5 μm) with mobile phase of acetonitrile-water at a flow rate of 1.0 ml/min,detection wavelength was 203 nm,column temperature was 30℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.199 8-3.996 0 μg for notoginsenoside R1,0.842 8-10.143 0 μg for ginsenoside Rg1 and 0.823 4-9.978 0 μg for ginsenoside Rb1;RSDs of precision,stability and reproducibility tests were low-er than 2%;recoveries were 95.17%-100.17%(RSD=1.81%,n=9),97.32%-101.18%(RSD=1.44%,n=9)and 95.22%-98.89%(RSD=1.22%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the simultaneous contents determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Shenqi xinshu capsule.
