Gene expression profiles analysis identifies key genes of PBMCs in patients with benign and malignant breast tumor
10.3969/j.issn.1000-484X.2016.10.004
- VernacularTitle:良恶性乳腺肿瘤外周血基因差异表达研究
- Author:
Lang HE
;
Na WEI
;
Zheng GUO
;
Dan WANG
- Publication Type:Journal Article
- Keywords:
Breast tumor;
PBMCs;
Differentially expressed gene;
Pathway enrichment analysis
- From:
Chinese Journal of Immunology
2016;32(10):1424-1427,1436
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of gene expression in peripheral blood mononuclear cells( PBMCs) of benign and malignant breast tumor based on gene expression profiling. Methods: Datasets of gene expression profiling were downloaded from the GEO database,including PBMCs profilings of benign breast tumor,breast cancer and healthy controls. GEO2R tool was used to analyze the data to identify the differentially expressed genes (DEGs). Function of DEGs were annotated by DAVID. Protein interaction analysis and hub gene select were then performed using STRING database. Results:563 and 237 DEGs respectively were identified. DEGs in breast cancer involved in biological process of leukocyte activation,angiogenesis and leukocyte transendothelial migration. The hub genes are IL8,RHOB,ITGB1. Conclusion:The data suggests that gene expression patterns of these two profilings are different at a certain degree. PBMCs maybe a better noninvasive material for biomarker detection of benign and malignant breast tumor.
