Effects of Tamsulosin on Proliferation and Apoptosis in Prostatic Cancer PC-3 Cells
10.6039/j.issn.1001-0408.2016.07.10
- VernacularTitle:坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响
- Author:
Ming ZHAO
;
Shan JIN
;
Jinhua HE
- Publication Type:Journal Article
- Keywords:
Tamsulosin;
Prostatic cancer PC-3 cells;
Proliferation;
Apoptosis;
Protein kinase B;
Mammalian target rapamycin
- From:
China Pharmacy
2016;27(7):896-898,899
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of tamsulosin on proliferation and apoptosis in prostatic cancer PC-3 cells. METHODS:After treated with 0 (blank control group),12.5,25 and 50 μmol/L tamsulosin (tamsulosin low,medium and high-concentration groups)for 48 h,the viability of PC-3 cells was detected by MTT method. Hoechst 33258 staining was used to detect cell apoptosis rate. Western blot was used to determine the expression level of Bax and Bcl-2 protein,and the phosphoryla-tion level of protein kinase B(Akt),mammalian target rapamycin(mTOR),ribosomal S6 protein kinase(p70S6K)and 4E bind-ing protein 1(4E-BP1). RESULTS:Compared with blank control group,PC-3 cells viability and the phosphorylation level of Akt, p70S6K and 4E-BP1 decreased in tamsulosin low,medium and high-concentration groups,while expression level of Bax protein in-creased (P<0.05 or P<0.01);the apoptosis rate of PC-3 cells was increased in tamsulosin medium and high-concentration groups,while the expression level of Bcl-2 and phosphorylation level of mTOR were decreased(P<0.01),in concentration-depen-dent manner. CONCLUSIONS:Tamsulosin can inhibit PC-3 cells proliferation and induce cell apoptosis via blocking Akt/mTOR signal pathway.
