Simultaneous Determination of Baicalin,Baicalein and Wogonin in Huatan Pingchuan Tablet by HPLC

VernacularTitle:HPLC法同时测定化痰平喘片中黄芩苷、黄芩素和汉黄芩素的含量
Author: Xiang CHEN ; Hongping ZHANG ; Jingjing XU
Publication Type:Journal Article
Keywords: HPLC; Huatan pingchuan tablet; Baicalin; Baicalein; Wogonin
From: China Pharmacy 2016;(6):846-847,848
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the simultaneous determination of baicalin,baicalein and wogonin in Hua-tan pingchuan tablet. METHODS:HPLC was performed on the column was Diamonsil C18 with mobile phase of methanol-0.1%phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 277 nm,column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.069 04-0.690 4 μg for baicalin(r=0.999 4),0.054 56-0.5456 μg for baicalein(r=0.999 6)and 0.030 36-0.303 6 μg for wogonin(r=0.999 1);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 97.64%-99.06%(RSD=0.57%,n=6),98.31%-100.64%(RSD=0.85%,n=6) and 97.53%-99.48%(RSD=0.76%,n=6). CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the contents determination of baicalin,baicalein and wogonin in Huatan pingchuan tablet.