Expression of miRNA -22 in esophageal and gastric cancer tissues and cell lines
10.3760/cma.j.issn.1008-6706.2016.22.011
- VernacularTitle:微小 RNA-22在食管癌和胃癌细胞及组织中的表达
- Author:
Lihua HU
;
Peng CHEN
;
Jianying ZHANG
;
Jiye ZHANG
;
Dongjie LIU
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Gastric neoplasms;
Cell lines;
microRNA -22
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(22):3404-3406,3407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical value of miRNA -22 in esophageal and gastric cancer tissues and cell lines.Methods Realtime -PCR was performed to detect the expression of miRNA -22 in human esophageal cancer cell lines ECA109,TE -1,TE -8,TE -13 and normal esophageal epithelial cells HEEC,48 cases of esophageal cancer tissues and matched adjacent normal tissues,human gastric cancer cell lines SGC -7901,MKN -45,NCI -N87,AGS,NUGC -3,SUN -1,normal human gastric mucosa cell line GES -1 and normal stomach fibroblastic cell NSFC,88 cases of gastric cancer tissues and matched adjacent normal tissues.Results The expression of miRNA -22 was much less in four esophageal cancer cell lines than that of immortalized normal esophageal epithelial cells HEEC,and the expression of miRNA -22 was much less in six gastric cancer cell lines than that of gastric mucosal epithelial cell line GES -1 and normal stomach fibroblastic cell line NSFC.The esophageal cancer tissues showed aberrant down regulation of miRNA -22 compared with the adjacent non -tumor tissues [(3.51 ±1.05)vs.(11.23 ±2.95),t =18.13,P <0.05].The gastric cancer tissues showed aberrant down regula-tion of miRNA -22 compared with the adjacent non -tumor tissues [(2.15 ±1.23)vs.(9.14 ±2.86),t =22.93, P <0.05].Conclusion The expression of miRNA -22 was much lower in esophageal and gastric cancer tissues and cell lines,which provided basement to nosogenesis,early diagnosis and create drug treatment of the cancers.
