Expression and antigenicity analysis of Clostridium difficile glutamate dehydrogenase
10.7644/j.issn.1674-9960.2016.09.004
- VernacularTitle:艰难梭菌谷氨酸脱氢酶的表达及抗原性分析
- Author:
Jianxia WANG
;
Xiqin YANG
;
Dazhi JIN
;
Chen HUANG
;
Yun LUO
;
Xiaoguo SONG
;
Hongwei WANG
;
Xiaoyan FENG
;
Heqiu ZHANG
- Publication Type:Journal Article
- Keywords:
Clostridium difficile;
glutamate dehydrogenase;
prokaryotic expression
- From:
Military Medical Sciences
2016;40(9):710-712,732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct prokaryotic expression vectors for glutamate dehydrogenase(GDH)of Clostridium difficile(CD),and express recombinant GDH in Escherichia coli,and identify its antigenicityed.Methods The entire gene of GDH was cloned from ATCC43255 genome DNA.The recombinant antigens were expressed in E.coli with IPTG induction and purified by Ni-NATBeads.The antigenicity was detected using CD Qick Chek Complete dual-antigen EIA. Results Prokaryotic expression vectors of CD GDH were constructed successfully.The antigen could be identified by specific anti-GDH antibodies.Conclusion The GDH antigen can be used to prepare corresponding antibodies,which facilitate the development of immunoassay for CD GDH.
