Expression of miR-203 in human tongue carcinoma tissues and its in-fluence on viability and invasion ability of Tca8113 cells
10.3969/j.issn.1000-4718.2016.10.026
- VernacularTitle:miR-203在舌癌组织中的表达及其对Tca8113细胞活力和侵袭的影响
- Author:
Hong ZHENG
;
Wenling ZHANG
;
Bo LIN
;
Sainan ZHANG
;
Ruimin LIU
;
Peng XUE
- Publication Type:Journal Article
- Keywords:
miR-203;
Tongue carcinoma;
Cell viability;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2016;32(10):1896-1899,1904
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To study the expression of miR-203 in tongue carcinoma tissues and the effect of miR-203 over-expression on the viability and invasion ability of Tca 8113 cells.METHODS:Twenty-eight pairs of tongue carcinoma tissues and adjacent nontumor tissues were collected , and the clinicopathological characters were analyzed .miR-203 was detected in the tongue tissues of 28 patients with tongue carcinoma by real-time PCR.miR-203 mimics and scramble were transfected into Tca8113 cells by Lipofectamine 2000.The expression of miR-203 was detected in Tca8113, Tca8113-miR-203 mimics and Tca8113-scramble cells by RT-qPCR.The cell viability was measured by CCK-8 assay.The cell invasion ability was determined by Transwell chamber invasion experiment .RESULTS:miR-203 expression was significantly down-regulated in the tongue carcinoma tissues compared with those in the adjacent nontumor tissues .The expression of miR-203 was associated with TNM stage and lymph node metastasis .Up-regulation of miR-203 inhibited the viability and invasion a-bility of Tca8113 cells.CONCLUSION:miR-203 suppresses the growth and invasion of tongue carcinoma cells .miR-203 may be a potential therapeutic target for treating human tongue cancer .
