The detection of the expression of CD44v8 with RT-PCR in bladder and urothelial carcinoma

VernacularTitle:实时荧光定量 PCR 检测 CD44v8在膀胱及尿路上皮癌中的表达
Author: Xiaorong LIU ; Yongxiang WANG ; Liming ZHAO ; Xiaodong ZHAN ; Xuejun LIU ; Yulin REN ; Shaojun LI
Publication Type:Journal Article
Keywords: carcinoma; bladder; urothelial carcinoma; RT-PCR; CD44v8 protein
From: International Journal of Laboratory Medicine 2016;37(19):2687-2688,2691
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of CD44v8 protein in human bladder and urothelial carcinoma ,as well as the value in diagnosis of human bladder and urothelial carcinoma .Methods RT‐PCR was used to analysis the expression of CD44v8 protein in 75 patients with bladder and urothelial carcinoma in the pathological stage and clinical stage and 20 subjects of normal bladder mucosa were collected as control .Results CD44v8 protein was negative in all normal bladder and urothelial mucosa ,the copy number was less than 1 × 102 copy/mL ;26 cases of bladder and urothelial carcinoma was positive ,and the positive rate of CD44v8 was 34 .7% ,and Ct values were less than 35 and copy number was greater than 1 × 104 copy/mL .Positive rate was correla‐ted with high pathological grades and TNM stages ,but no significant difference was observed in recurrence of tumor .Conclusion CD44v8 could be useful indicator for the assessment of pathological grades and TNM stages of bladder and urothelial carcinoma .