Change of phenotype of stromal fibroblasts in esophageal carcinogenesis
10.3969/j.issn.1671-8348.2016.27.010
- VernacularTitle:食管癌变进程中间质成纤维细胞的表型变化
- Author:
Zhibin XU
;
Li YUAN
;
Xiuli ZHENG
;
Shijie WANG
;
Mingli WU
- Publication Type:Journal Article
- Keywords:
esophageal neoplasms;
stromal cells;
stromal fibroblast;
carcinogenesis;
phenotype
- From:
Chongqing Medicine
2016;45(27):3777-3779,3782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions ofα‐SMA in different esophageal stromal fibroblasts in esophageal car‐cinogenesis .Methods IHC method was uesd to detect the expression of α‐SMA protein in stromal fibroblasts of twenty normal e‐sophageal tissues ,eighty precancerous lesions and fifty esophageal carcinomas respectively .Three kinds of esophageal stromal fibro‐blasts were cultured primarily and cells immunohistochemical staining was carrired out after being purified .Expression of α‐SMA was detected by RT PCR .Results IHC results showed thatα‐SMA expressions in normal ,precancerous and cancerous lesions were of significant differences .RT‐PCR results showed thatα‐SMA expressions were different significantly among three kinds of fibro‐blasts .Conclusion Esophageal stromal fibroblasts were activated with carcinogenensis .AFs was possibly the origion of CAFs .
