Simultaneous Determination of Paeoniflorin and Paeonol in Dieda Pill by HPLC
10.6039/j.issn.1001-0408.2016.27.42
- VernacularTitle:HPLC法同时测定跌打丸中芍药苷和丹皮酚的含量
- Author:
Hongyun DOU
;
Dongjie HUANG
;
Mengyang WANG
;
Jie ZHANG
;
Xiaoqi WANG
;
Yongqing TIAN
- Publication Type:Journal Article
- Keywords:
Dieda pill;
Paeoniflorin;
Paeonol;
Content determination;
HPLC
- From:
China Pharmacy
2016;27(27):3870-3871,3872
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin and paeonol in Dirda pill, and provide reference for improving its quality control standard. METHODS:HPLC was performed on the column of ZORBAX C18 with mobile phase of acetonitrile-0.1% phosphoric acid (gradient elutio) at a flow rate of 0.8 ml/min,detection wavelength was 230 nm(paeoniflorin),274 nm(paeonol),columne temperature was 35 ℃,injection volume was 20 μl. RESULTS:The linear range was 0.514 8-1.544 5μg for paeoniflorin(r=0.999 2)and 0.643 2-1.960 4μg for paeonol(r=0.999 8);the limits of quantifi-cation were 0.135 6,0.126 4 μg,limits of detection were 0.067 8,0.063 2 μg;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.78%-97.27%(RSD=0.62%,n=6) and 97.38%-98.38%(RSD=0.42%,n=6). CONCLUSIONS:The method is simple with good reprosucibility and high sensibility,and can be used for the simultaneous deter-mination of paeoniflorin and paeonol in Dirda pill.
