Simultaneous Determination of Scutellarin and Linarin in Zhuang Medicine Scoparia dulcis by HPLC
10.6039/j.issn.1001-0408.2016.27.33
- VernacularTitle:HPLC法同时测定壮药冰糖草中野黄芩苷和蒙花苷的含量
- Author:
Wenfang MA
;
Huilian DENG
;
Yurong TANG
;
Yi CAI
- Publication Type:Journal Article
- Keywords:
Scoparia dulcis;
Scutellarin;
Linarin;
HPLC
- From:
China Pharmacy
2016;27(27):3845-3846,3847
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of scutellarin and linarin in Scoparia dulcis. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile-0.05% phosphoric acid (gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 330 nm,column temperature was 30 ℃,and the in-jection volume was 10 μl. RESULTS:The linear range was 0.015 8-0.316 8 mg/ml for scutellarin(r=0.999 9)and 0.000 5-0.010 4 mg/ml for linarin (r=0.999 7);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 99.56%-102.38%(RSD=1.07%,n=6)and 95.14%-98.29%(RSD=1.24%,n=6),respectively. CONCLUSIONS:The method is simple with good stability and reprodicibility,and can be used for the simultaneous determination of scutellarin and linarin in S. du-lcis.
