Content Determination of Icariin in Kangguzhi Zengsheng Tablet by HPLC
10.6039/j.issn.1001-0408.2016.30.37
- VernacularTitle:HPLC法测定抗骨质增生片中淫羊藿苷的含量
- Author:
Guilin ZHANG
;
Huiping MA
;
Lanru LI
;
Zhichen DONG
;
Lei HE
;
Zhengping JIA
- Publication Type:Journal Article
- Keywords:
Kangguzhi zengsheng tablet;
Icariin;
HPLC;
Content determination
- From:
China Pharmacy
2016;27(30):4291-4292,4293
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of icariin in Kangguzhi zengsheng tablet,and pro-vide a basis for improving the quality standard. METHODS:HPLC was performed on the column of Hypersil ODS2 C18 with mo-bile phase of acetonitrile- water(27∶73,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 270 nm,column temperature was 25℃,and injection volume was 10μl. RESULTS:The linear range of icariin was 3-60μg/ml(r=0.999 9);limit of quantita-tion was 11.2 μg/ml,limit of detection 4.3 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.0%;re-covery was 99.00%-100.67%(RSD=0.63%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the content determination of icariin in Kangguzhi zengsheng tablet.
