Content Determination of Peoniflorin in Danggui Shaoyao Powder by HPLC
10.6039/j.issn.1001-0408.2016.30.25
- VernacularTitle:HPLC法测定当归芍药散中芍药苷的含量
- Author:
Jun ZHOU
;
Deng WANG
;
Li ZHANG
;
Jin ZHANG
;
Maoxing LI
;
Ruxue ZHANG
;
Zhengping JIA
- Publication Type:Journal Article
- Keywords:
Danggui shaoyao powder;
Peoniflorin;
HPLC;
Content determination
- From:
China Pharmacy
2016;27(30):4255-4256,4257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of peoniflorin in Danggui shaoyao powder,and provide a reference for controlling the quality of the preparation. METHODS:HPLC was performed on the column of Symmetry C18 with mobile phase of acetonitrile-water(containing 0.1% phosphoric acid)(14∶86,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 20℃,and injection volume was 20μl. RESULTS:The linear range of peoniflo-rin was 10-80 μg/ml(r=0.999 3);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 98.3%-104.9%(RSD=2.0%,n=9). CONCLUSIONS:The method is simple,accurate and specific,and can be used for the con-tent determination of peoniflorin in Danggui shaoyao powder.
