Effects of downregulation of CD147 in the mobility of thyroid cancer cell SW579 and its mechanisms
10.3969/j.issn.1006-5725.2016.20.015
- VernacularTitle:下调CD147表达对甲状腺癌细胞SW579运动能力的影响
- Author:
Wei WANG
;
Yue SHAO
;
Xiaowen SONG
;
Chenghai ZHAO
- Publication Type:Journal Article
- Keywords:
SW579 cells;
CD147;
siRNA;
Mobility;
WAVE 2
- From:
The Journal of Practical Medicine
2016;32(20):3335-3337,3338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effects and mechanisms of CD147 in thyroid cancer cell SW579. Methods RT-PCR and Western-blot were used to determine the levels of CD147 mRNA and protein in SW579 cells after treated with CD147 specific small interference RNA (siRNA). The proliferative ratio of SW579 cells after treated with CD147 siRNA was detected by CCK-8 assay. We detected the changes of cellular mobility after treated with CD147 siRNA by transwell assay. Mobility related proteins were analyzed by western-blot. Results The proliferative ratio and the mobility of SW579 cells were inhibited significantly by down-regulation of CD147. The levels of MMP-2 and MMP-9 were lower in treated cells than untreated ones. Expression of Wave 2 and a mobility related protein were decreased in accompany with down-regulation of CD147. Conclusion Down-regulation of CD147 inhibits mobility of SW579 by suppressing MMP-2, MMP-9 and WAVE 2.
