Content Comparison of 18 Amino Acids in Plancenta Histolysate Determined by Post-column Derivatization Cation-exchange Chromatography and Pre-column Derivatization HPLC
- VernacularTitle:柱后衍生阳离子交换色谱法与柱前衍生高效液相色谱法测定人胎盘组织液中18种氨基酸含量比较
- Author:
Yuanqing LI
;
Yi BAO
- Publication Type:Journal Article
- Keywords:
Pre-column derivatization;
HPLC;
Post-column derivation;
Cation-exchange chromatography;
Amino acid
- From:
China Pharmacist
2016;19(10):1830-1833,1846
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the content differences of 18 amino acids in plancenta histolysate determined by pre-column de-rivatization HPLC and post-column derivatization cation-exchange chromatography ( AARO) . Methods: The HPLC method was per-formed on a C18 column and 2, 4-dinitro chlorobenzene ( DNCB) was used for pre-column derivatization, and then the determination was carried out after adding 0. 1 mol·L-1 borax buffer (pH=9. 1), and the AARO method was used for the direct determination with a strong acid cation-exchange chromatographic column and post-column derivatization. Results: The RSD for reproducibility of the AARO method was 1. 84%-0. 91%, while that of the HPLC method was 1. 87%-1. 04%. Conclusion:Both AARO method and HPLC method can be used for the quantitative determination of 18 amino acids in plancenta histolysate with the similar results. However, pre-column derivatization HPLC method may cause incomplete derivatization and instable derivatives.
