The in vitro and in vivo models induced by shrimp tropomyosin and monoclonal tropomyosin-specific murine IgE antibody

VernacularTitle:虾原肌球蛋白及其IgE单克隆抗体建立I型过敏反应体内外模型
Author: Lei FANG ; Rui HOU ; Qiaoling FEI ; Yuan GAO ; Fen LIU ; Runlan CAI ; Yun QI
Publication Type:Journal Article
Keywords: type Ⅰ allergy; tropomyosin; Metapenae-usensis; monoclonal antibody; IgE; RBL-2 H3 cells
From: Chinese Pharmacological Bulletin 2016;32(10):1422-1426,1427
CountryChina
Language:Chinese
Abstract: Aim To develop the in vitro and in vivo models induced by shrimp tropomyosin( ST) and mono-clonal tropomyosin-specific murine IgE antibody ( anti-ST-IgE mAb) . Methods ST was purified from Metap-enaeusensis by an isoelectric precipitation method. The anti-ST-IgE mAb was obtained from hybridomas. After RBL-2 H3 cells were sensitized with anti-ST-IgE mAb and challenged with ST,β-hexosaminidase release was determined. Passive systemic anaphylaxis ( PSA ) was induced in mice and the rectal temperature was recor-ded after ST challenge within 30 min by a thermal probe. Results A significant increase ofβ-hexosamin-idase was observed in sensitized cells after ST chal-lenge. The average temperature drop after ST challenge was 1. 44℃ in PSA mice within 30 min. Conclusion The in vitro and in vivo models induced by ST and anti-ST-IgE mAb are established as an improvement of pres-ent models of type Ⅰ allergy.