Study on the change of migration ability of dendritic cells induced by intermittent hypoxia and the mechanism of its pathway
10.3969/j.issn.1671-8348.2016.30.008
- VernacularTitle:间歇性低氧致树突状细胞迁徙能力改变及其通路机制探讨
- Author:
Ke HU
;
Guangwei WANG
;
Yu YANG
;
Guoyao XU
- Publication Type:Journal Article
- Keywords:
intermittent hypoxia;
dendritic cells;
migratory ability;
RelB;
p38
- From:
Chongqing Medicine
2016;45(30):4200-4202,4206
- CountryChina
- Language:Chinese
-
Abstract:
Objective By simulating the intermittent hypoxia(IH ) environment of human obstructive sleep apnea syndrome (OSAS) ,to reveal the effect of IH on migratory ability of human peripheral blood derived dendritic cells(DCs) ,and through the in‐tervention of RelB ,p38 expression in order to explore the possible mechanism of the change of DCs migration ability induced by IH . Methods DCs were divided into RelB ,p38 siRNA interfering and non interfering plasmid group before cultivation .The environment of hypoxia was created by a intermittent hypoxia cabin ,among them ,oxygen concentration was 0 .5% ,1 .5% ,5 .0% ,10 .0% ,hypox‐ia/reoxygenation time ratio was set as 1∶1 ,1∶3 ,1∶5 and 1∶9 ,while sustained oxygen was supplied to the contrast at a normal concentration of 21 .0% .The content of RelB and p38 was tested by Western blotting after culture in vitro ,migration ability of DCs was detected by invasion chamber .Results Compared with normoxia ,DCs under IH tended to have declined migratory ability , which was confirmed to be correlated with the average oxygen partial pressure level under IH .IH could promote the expression of RelB and p38 in DCs ,while the migratory ability of DCs was not reversed after intervening the expression of RelB and p38 .Conclu‐sion IH in vitro could cause a decline in migratory ability of DCs ,which may not be induced by activation of RelB or p38 in DCs .
