Role of Relaxin-2 in the migration of human vascular smooth muscle cells and its underlying mechanisms
10.3969/j.issn.1671-8348.2016.30.010
- VernacularTitle:H2型松弛素促进人血管平滑肌细胞迁移的作用及机制
- Author:
Xia WANG
- Publication Type:Journal Article
- Keywords:
Relaxin-2;
vascular smooth muscle cells;
migration
- From:
Chongqing Medicine
2016;45(30):4207-4210,4213
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and the associated mechanism of Relaxin‐2 in the migration of human vascular smooth muscle cells (VSMCs) .Methods The migration of VSMCs in responsing to Relaxin‐2 was evaluated by using wound heal‐ing assay and transwell assay ,and the cell signaling proteins ,including Akt ,ERK and NF‐κB p65 ,in responsing to Relaxin‐2 were measured by using Western blotting assay .Results Relaxin‐2 can promote the migration of VSMCs in a dose‐dependent manner , NF‐κB inhibitor BAY11‐7082 can block the expression of MMP‐9 and MMP‐2 induced by Relaxin‐2 ,pretreatment with PI3K inhibi‐tor LY294002 and ERK inhibitor U0126 can reduce the activation of NF‐κB p65 induced by Relaxin‐2 .Conclusion NF‐κB p65 could be activated by the activation of PI3K/Akt and ERK signaling pathway induced by Relaxin‐2 ,thereby promoting the expression of MMP‐9 and MMP‐2 ,and inducing VSMCs migration effect .
