Expression and Its Significance of MicroRNA-1 4 5 and MicroRNA-1 4 3 in Plasma in Children with Kawasaki Disease
10.3969/j.issn.1671-7414.2016.05.009
- VernacularTitle:miRNA-145和miRNA-143在川崎病患儿血浆中的表达及意义
- Author:
Weiwu SHENG
;
Chunbiao GAO
;
Hualing GUAN
- Publication Type:Journal Article
- Keywords:
kawasaki disease;
miRNA-145;
miRNA-143
- From:
Journal of Modern Laboratory Medicine
2016;31(5):34-37,41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of the quantitative detection of miRNA-145 and miRNA-143 in the ser-um of children with Kawasaki disease (KD).Methods In this study,45 KD cases were enrolled and were divided into 19 ca-ses with coronary artery lesions (CAL group)and 26 cases without coronary artery lesions (NCAL group).Thirty healthy children were recruited as the control group (NC group).qRT-PCR was conducted to detect the expression of miRNA-145 and miRNA-143 in serum of each group.The diagnostic value of miRNA-145 and miRNA-143 were evaluated by receiver op-erating characteristic curves (ROC)and the area under the curve (AUC)(95%CI).Results The relative expressions of miRNA-145 and miRNA-143 in the serum of the CAL group in acute phase were 2.33±1.26 and 1.64±0.50,which were respectively higher than the control group,with statistically significant difference (t=5.108,5.072,P<0.01).The relative expression of miRNA-145 during the recovery phase of CAL group was 1.55±0.49,which was statistically significant high-er than NC group (t=3.837,P<0.01).While the relative expression of miRNA-143 during the recovery phase of CAL group was 1.17±0.33 and had no difference with NC group (t=1.033,P>0.05).During the acute phase of NCAL group, the relative expressions of miRNA-145 and miRNA-143 were 2.02±1.00 and 1.63±0.50 respectively.They were signifi-cantly higher than the control group (t=4.746,5.261,P<0.01).However,when comes to the recovery phase,the relative expression of miRNA-145 and miRNA-143 were 1.07±0.18 and 1.12±0.16,which had no difference with NC group (t=0.264 9,0.584 9,P>0.05).The critical value of miRNA-145 for diagnosis of KD was 1.697 with sensitivity of 64.44% and specificity of 90% and yielded an area under the curve of ROC of 0.7733 (95%CI:0.667 0~0.879 7).Also,the critical val-ue of miRNA-143 was 1.361 with sensitivity of 64.44% and specificity of 86.67% and yielded an area under the curve of ROC of 0.8163 (95%CI:0.722 5~0.910 0)in discriminating KD from healthy group.Conclusion miRNA-145 and miR-NA-143 may prove to be a non-invasive biomarker for the auxiliary diagnosis of KD.
