The clinical application to detect the anti cyclic citrullinated peptide antibody by immune Turbidimetry
10.3969/j.issn.1673-4130.2016.20.021
- VernacularTitle:免疫比浊法测定抗环瓜氨酸肽抗体的临床应用
- Author:
Haoquan CHEN
;
Manni ZENG
- Publication Type:Journal Article
- Keywords:
immunoturbidimetry;
rheumatoid arthritis;
anti cyclic citrullinated peptide antibody
- From:
International Journal of Laboratory Medicine
2016;37(20):2857-2858,2861
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical application value of anti cyclic citrullinated peptide antibody(CCP) by immune tur‐bidity method for the the diagnosis of rheumatoid arthritis (RA) .Methods The serum of 195 cases of RA patients ,105 cases of os‐teoarthritis ,52 cases of osteoporosis ,42 cases of connective tissue ,40 patients with systemic lupus erythematosus ,305 cases of pa‐tients with other diseases and 90 cases of healthy controls were collected ,the CCP were detected by latex CMIA and ELISA . Results The CCP positive were 141 cases in RA(72 .30% ) ,54 cases(51 .42% ) in arthritis ,15 cases in osteoporosis (28 .84% ) ,15 cases in connective tissue(35 .71% ) ,18 cases (45 .0% ) in systemic lupus erythematosus (SLE) ,11 cases (3 .60% ) in other disea‐ses .There were negative in 90 cases of healthy controls .The variation coefficient was 1 .9% -2 .0% ,and the total coefficient of var‐iation was 2 .0% -2 .1% .The linear range of Latex method was divided into 0 .8-100 U/mL .There were no diffrence in the sensi‐tivity and specificity of detection to CCP by CMIA ,ELISA and Latex .Conclusion The sensitivity and specificity of detection to CCP by Latex was good ,and there was wide linear range .
