The expression of miR-200b and CEA and the correlation between the two factors in colon cancer
10.3969/j.issn.1673-4130.2016.20.002
- VernacularTitle:miR-200b与癌胚抗原在大肠癌中的表达及其相关性
- Author:
Hailian WANG
;
Lili YIN
;
Chunmei WU
;
Jinhua MIAO
- Publication Type:Journal Article
- Keywords:
colon cancer;
miR-200b;
CEA;
real-time PCR
- From:
International Journal of Laboratory Medicine
2016;37(20):2808-2809,2812
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of transcription factor miR‐200b and CEA and the correlation between the two transcription factors in the process of occurrence and development of colorectal cancer .Methods Expression miR‐200b and CEA mRNA by real‐time PCR in 60 colon cancer tissues and corresponding normal tissues were detected and the results were compared with the clinical features and pathological characters .The relationship between the mRNA expression of miR‐200b and CEA in 60 colon cancer tissues was determined .Results The expression rate of CEA mRNA was detectable to highly expressed rates in colon cancer tissues than that in matched normal tissues (P<0 .01) ,whereas miR‐200b mRNA in the colon cancer tissues was signifi‐cantly lower than that in the matched normal tissues(P<0 .01) .There was no significant correlation between miR‐200b ,CEA mR‐NA expression and age ,sex ,tumor location(P>0 .05) .Loss expression or down regulation expression of miR‐200b mRNA was de‐tected ,whereas CEA mRNA was detectable to highly expressed in the different histological grade and Dukes stages .The expression of miR‐200b mRNA and CEA mRNA were positively correlated with the histological grade in colon cancer .A significant correlation was found between the expression of miR‐200b mRNA and CEA mRNA (r= -0 .790 ,P<0 .001) .Conclusion Loss or down regu‐lation expression of miR‐200b mRNA ,whereas CEA is detectable to highly expressed in colon cancer .Negative correlation occurred in miR‐200b mRNA and CEA mRNA indicates that miR‐200b and CEA provide the new clues of genetic diagnosis and treatment .
