Study on microtiter plate kinetic method to detection ALT
10.3969/j.issn.1673-4130.2016.20.015
- VernacularTitle:微板速率法检测丙氨酸氨基转移酶的研究
- Author:
Zhengyu LIU
;
Xueqin YANG
;
Changye HUI
- Publication Type:Journal Article
- Keywords:
ALT;
microtiter plate kinetic method;
enzyme linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2016;37(20):2841-2842,2846
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical application of ALT detection based on microtiter plate kinetic method in the au‐tomatic enzyme immunoassay system .Methods By beference to the IFCC recommended kinetic method ,microtiter plate kinetic method was established in the automatic enzyme immunoassay system of ALT detection .The linear ,intro‐batch and inter‐batch du‐plicability of the method were evaluated .ALT test results of 823 samples with microtiter plate kinetic method and automatic bio‐chemistry analyzer method were compared .Results Microtiter plate kinetic method had good linearity where ALT activity <303 U/L ,the intra batch and inter batch variation coefficients < 1/5TEa .The detection results of the clinical samples were correlated with the biochemical analyzer .Conclusion Microtiter plate kinetic method to detection ALT in the automatic enzyme immunoassay system is an ideal method for simultaneous detection of ALT and ELISA in large batch samples ,which is worth popularizing .
