Content Determination of Heptanone in Mongolian Medicine Compound Shudage-4 by HPLC
10.6039/j.issn.1001-0408.2015.27.29
- VernacularTitle:HPLC法测定蒙药复方述达格-4中庚酮类成分的含量
- Author:
Shasha XIN
;
Yang YANG
;
Guoyuan SUN
;
Yu DONG
- Publication Type:Journal Article
- Keywords:
Mongolian medicine;
Compound shudage-4;
1-phenyl-7-(3-methoxy-4-hydroxy)phenyl-5-ol-3-heptanone;
HPLC;
Content determination
- From:
China Pharmacy
2015;(27):3826-3827,3828
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of 1-phenyl-7-(3-methoxy-4-hydroxy) phe-nyl-5-ol-3-heptanone in active ingredients of Mongolian medicine compound shudage-4. METHODS:HPLC was performed on the column of Intersil ODS-3 with the mobile phase of acetonitrile-0.5%phosphoric acid at the flow rate of 1.0 ml/min,the detection wavelength was 210 nm,temperature was 30 ℃,and the volume was 20 μl. RESULTS:The linear range of 1-phenyl-7-(3-me-thoxy-4-hydroxy) phenyl-5-ol-3-heptanone was 0.102-1.02 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.52%;average recovery was 97.10%(RSD=1.80%,n=6). CONCLUSIONS:The method is accurate,sim-ple and reproducible,and can provide basis for the quality control of Mongolian medicine compound shudage-4.
