Study on Inhibitory Effects Mechanism of Scallop Skirt Glycosaminoglycan on Oxidative Stress Injury in Vein Endothelium Cells Induced by OX-LDL
10.6039/j.issn.1001-0408.2015.28.17
- VernacularTitle:扇贝糖胺聚糖对OX-LDL诱导血管内皮细胞氧化应激损伤的抑制作用机制研究Δ
- Author:
Chuanxia JU
;
Lin HOU
;
Peiyang CONG
;
Jiayi WANG
;
Fusheng SUN
;
Lei WANG
;
Fang ZHANG
;
Sai LIU
- Publication Type:Journal Article
- Keywords:
Scallop skirt glycosaminoglycan;
Human umbilical vein endothelium cell;
Reactive oxygen species;
Lectin-like ox-idized low-density lipoprotein receptor-1;
NADPH oxidase
- From:
China Pharmacy
2015;(28):3938-3940,3941
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effects mechanism of scallop skirt glycosaminoglycan(SS-GAG)on inju-ry in human umbilical vein endothelium cells (HUVEC). METHODS:In the test,there was a negative control group,a model group and the groups of SS-GAG at high,middle and low concentrations(mass concentrations of 200,100 and 50 mg/L respective-ly). The cells in latter 3 groups were cultured in SS-GAG at different mass concentrations for 12 h,and then in 50 μmol/L oxidized low-density lipoprotein(OX-LDL)for 24 h. MTT method was used to detect cell viability and the activity of lactic dehydrogenase (LDH),the flow cytometer to determine the level of reactive oxygen species (ROS),real-time fluorescence quantitative poly-merase chain reaction (RT-PCR) to detect mRNA expression of lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1), and Western blot to detect NOX4 protein expression. RESULTS:Compared to the cells in the negative control group,those in the model group demonstrated lower viability,higher activity of LDH,higher level of ROS,and stronger expressions of LOX-1 mRNA and NOX4 protein. There was statistical significance (P<0.01). Compared to the cells in the model group,those in the groups of SS-GAG at high,middle and low concentrations showed higher viability,lower activity of LDH,lower level of ROS and weaker expressions of LOX-1 mRNA and NOX4 protein. There was statistical significance (P<0.01). CONCLUSIONS:SS-GAG can protect HUVEC to some degree by a mechanism which may be related to inhibiting ROS production via LOX-1/NOX4 pathway and relieving oxidative stress injury.
