Preparation and Formula Optimization of Silybin Liposomes Coated by N-trimethyl Chitosan
10.6039/j.issn.1001-0408.2015.28.28
- VernacularTitle:N-三甲基壳聚糖包覆的水飞蓟宾脂质体的制备及处方工艺优化
- Author:
Wen HE
;
Cuiping HU
;
Zhen QU
- Publication Type:Journal Article
- Keywords:
Silybin;
N-trimethyl chitosan;
Liposome;
Orthogonal test;
Film dispersion method;
Multiple emulsion method;
Reverse evaporation method
- From:
China Pharmacy
2015;(28):3974-3976,3977
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare silybin liposomes(SLBL)coated by N-trimethyl chitosan(TMC)(TMC60-SLBL),and to optimize the formula. METHODS:The effects of 3 kinds of preparation methods on encapsulation efficiency of SLBL were com-pared,including film dispersion method,multiple emulsion method and reverse evaporation method. The formula of TMC60-SLBL was optimized by orthogonal test using encapsulation efficiency as index with the concentration of phospholipid,ratio of phospholip-id to cholesterol,ratio of silybin to lipids,hydration temperature as factors. The stability of TMC60-SLBL by optimal formula at 4 and 25℃within 30 d,the particle size and Zeta potential of TMC60-SLBL and SLBL were all compared. RESULTS:The encapsu-lation efficiency of TMC60-SLBL prepared by the film dispersion method was the highest,without statistical significance in encap-sulation efficiency before and after cutting (P>0.05). The optimal formula was with the concentration of phospholipid 6 mg/ml;the ratio of phospholipid to cholesterol 40∶1;the ratio of silybin to lipids 1∶30;the temperature of hydration medium 45 ℃;the encapsulation efficiency were(82.08±2.6)%,RSD=3.17%(n=3). The preparation was stable at 4℃;the mean diameter of SL-BL and TMC60-SLBL were(131.9±1.9)nm and(161.2±2.0)nm,and Zeta potential respectively were(-23.18±1.14)mV and (36.73 ± 2.84) mV. CONCLUSIONS:TMC60-SLBL is prepared successfully,and its formula is simple and practicable with high encapsulation efficiency.
