Evaluation of Quality Coherence of Commercially Available Artemisia apiacea Decoration Pieces in Chongq-ing by HPLC Fingerprint Combined with Common Model Recognition
10.6039/j.issn.1001-0408.2015.27.30
- VernacularTitle:HPLC指纹图谱结合共有模式识别评价重庆市售青蒿饮片质量的一致性
- Author:
Chengyou JIA
;
Fengping ZHAO
;
Wei LI
;
Jiao CHEN
;
Rongping YANG
;
Yunhong WANG
- Publication Type:Journal Article
- Keywords:
Artemisia apiacea;
HPLC;
Fingerprint;
Principal component analysis;
Cluster analysis
- From:
China Pharmacy
2015;(27):3828-3830,3831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the fingerprint of Artemisia apiacea to identify common model and evaluate the quality coherence of the commercially available A. apiacea decoration pieces in Chongqing. METHODS:HPLC method was performed on the column of Waters XTerra C18 with the mobile phase consisted of methanol-0.1% phosphoric acid(gradient elution)at the flow rate of 1.0 ml/min. The detection wavelength was 220 nm,column temperature was 30 ℃ and the sample size was 10 μl. The simi-larity of 12 batches of samples was evaluated by TCM Chromatogram Fingerprint Similarity Evaluation System(2004A edition);a common model of fingerprints was identified by principal component analysis(PCA)and cluster analysis(CA). RESULTS:Total-ly 11 batches of samples were screened to establish common model of fingerprints and 16 common peaks were obtained,among which,3 common components were identified;RSDs of precision,stability and reproducibility tests were lower than 2.53%. The similarity of 11 batches of A. apiacea and reference chromatogram was more than 0.990. CONCLUSIONS:The method is stable and easy,and can provide reference for the scientific quality evaluation and control of A. apiacea;the quality of commercially available A. apiacea decoration pieces is generally good,however,there is still a little number of batches with poor quality. The production procedures and supervision of A. apiacea decoration pieces should be strengthened.
