Simultaneous Determination of the Contents of Geniposide and Liquiritin in Xinshenning Tablets by HPLC
10.6039/j.issn.1001-0408.2015.27.42
- VernacularTitle:HPLC法同时测定心神宁片中栀子苷和甘草苷的含量
- Author:
Jia SONG
;
Shuqing DUAN
;
Yunxia LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Xinshenning tablets;
Geniposide;
Liquiritin;
Content
- From:
China Pharmacy
2015;(27):3862-3863,3864
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of the contents of geniposide and liquiritin in Xinshenning tablets. METHODS:HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water (gradient elution) at flow rate of 1.0 ml/min,the column temperature was 25 ℃,detection wavelength was 238 nm and the vol-ume was 10 μl. RESULTS:The mass concentration range was 16.3-407.9 μg/ml for geniposide(r=0.999 9) and 7.4-185.9 μg/ml for liquiritin(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.63%;average recoveries were 99.05%(RSD=1.28%,n=9)and 98.00%(RSD=1.84%,n=9),respectively. CONCLUSIONS:The established method is accu-rate,sensitive and simple,and can be used for the contents determination of geniposide and liquiritin in Xinshenning tablets.
