Cellular biocompatibility and biomechanical properties of 3D printed tracheal graft
10.3760/cma.j.issn.1673-4181.2016.04.002
- VernacularTitle:3D打印气管补片细胞相容性和生物力学性能研究
- Author:
Yao WANG
;
Yibo SHAN
;
Hongcan SHI
;
Junfeng YANG
;
Yiwei FAN
- Publication Type:Journal Article
- Keywords:
3D printed trachea graft;
Biomechanical properties;
Cellular biocompatibility;
Tissue-engineered trachea
- From:
International Journal of Biomedical Engineering
2016;39(4):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare 3D printed tracheal graft and investigate its cellular biocompatibility and biomechanical properties.Methods Bone marrow was isolated from tibial plateau of young New Zealand white rabbit,and bone mesenchymal stem cells (BMSCs) were obtained by whole bone marrow culture method and adherent purification method.Biomechanical test was performed for 3D printed trachea graft.After co-cultured of 3D printed trachea graft and BMSCs,cell morphology was observed and the proliferation index of the cells on 3D printed trachea graft was quantified using sulforhodamine B (SRB) assay.Results 3D printed trachea graft showed excellent biomechanical properties.Cell morphology was normal and cells grew well after co-culture with 3D printed trachea graft.The SRB assay indicated good proliferation of BMSCs on 3D printed trachea graft.Conclusions 3D printed trachea graft shows favorable cellular biocompatibility and biomechanical properties,and therefore can be used as a scaffold material for tissue-engineered trachea.
