Golgi dispersal in irradiated cells and the protective effect of vanillin derivatives
10.7644/j.issn.1674-9960.2016.10.008
- VernacularTitle:放射损伤细胞中高尔基体弥散现象及香兰素衍生物的防护作用
- Author:
Shudong YAN
;
Hua GUAN
;
Yu WANG
;
Xiaodan LIU
;
Pingkun[ ZHOU
- Publication Type:Journal Article
- Keywords:
radiation injuries;
Golgi dispersal;
vanillin derivative;
radiation protection;
cell cycle;
Gamma rays;
DNA damage
- From:
Military Medical Sciences
2016;40(10):809-813,857
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the Golgi dispersal in radiation damaged cells and the protective effect of vanillin derivatives.Methods Immunofluorescence, cell cycle analysis of flow-cytometry,Western blot,and clone formation were used.Results Immunofluorescence observation showed that the Golgi dispersal caused by 2 Gy 60 Coγ-ray was significantly increased in a dose-dependent manner in the range of 4-10 Gy as was demonstrated by the fact that the Golgi area was significantly increased. When the irradiated cells were treated with the radioprotective agent VND3207, a vanillin derivative,the Golgi dispersal induced by radiation was significantly reduced.The radiation-induced Golgi dispersal was also displayed in a pattern of time-course after irradiation in the HeLa cells, and persisted at least to 36 h post-irradiation. Cell cycle test results indicated that the Golgi dispersal was not associated with the G2/M arrest triggered by radiation-induced DNA damage response.VND3207 could promote cell survival by plate colony formation assay.Conclusion The Golgi dispersal can be caused byγ-ray irradiation in a dose-and time-dependent manner, and VND3207 can provide a good protection against radiation injury associated with inhibited Golgi dispersal.
