Observation of the estrous cycle in female NOD/SCID mice
10.3969/j.issn.1005-4847.2016.05.016
- VernacularTitle:NOD／SCID雌性小鼠动情周期的观察
- Author:
Yuhua ZHEN
;
Yang SONG
;
Yongge GUAN
;
Kunyin LI
;
Guangyun HU
;
Huihui LIAO
- Publication Type:Journal Article
- Keywords:
NOD /SCID mice;
Estrous cycle;
Vaginal smear;
Ovariectomy
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(5):526-528,545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in estrous cycle and vaginal smears in ovarectomized NOD/SCID mice.Methods To continuously observe the estrous cycle time by vaginal smears of NOD/SCID mice in consecutive nine days, twice daily.After ovariectomy, the changes of estrous cycle were observed by vaginal smears for 7 days.Results The estrous cycle in NOD/SCID mice was 4-6 days.Regular estrous mice accounted for 80%.There was no significant correlation between vaginal opening and estrous cycle status.After ovariectomy, the vaginal smears showed characteristics of metestrus or diestrus.Conclusions Vaginal smear cytology can be used to determine the estrous cycle and characteris-tics of NOD/SCID female mice.The ovariectomized operation of NOD/SCID female mice is effective.
