A metabolomics study on Shen-Mai decoction’ s protection of myocardial injured rats
10.3969/j.issn.1001-1978.2016.11.016
- VernacularTitle:参麦方对于大鼠心肌损伤保护作用的代谢组学研究
- Author:
Jie LI
;
Chao HU
;
Longlong XU
;
Zengchun MA
;
Qiande LIANG
;
Xianglin TANG
;
Yuguang WANG
;
Hongling TAN
;
Chengrong XIAO
;
Yue GAO
- Publication Type:Journal Article
- Keywords:
Shen-Mai decoction;
UPLC/TOF-MS;
metabolomics;
doxorubicin;
myocardial injury;
mecha-nism
- From:
Chinese Pharmacological Bulletin
2016;32(11):1559-1564,1565
- CountryChina
- Language:Chinese
-
Abstract:
Aim With metabolomics method, to study Shen-Mai decoction’ s function on protecting the myo-cardial injured rats caused by doxorubicin for probing into the functioning mechanism of Shen-Mai decoction’ s medical effect. Methods By means of UPLC-TOF-MS, the metabolites of urine of the rats treated by Shen-Mai decoction were analyzed. Then, the differ-ences between each group of the metabolites were sought with PLS-DA ( the partial least square discrimi-nant analysis ) and OPLS-DA ( the orthogonal partial least squares discriminant analysis ) . VIP ( variable importance in projection ) and t test were used to screen out potential biomarkers. Results Fourteen endogenous metabolites such as succinyladenosine, a-denosine 2′, 3′-cyclic phosphate, S-( 3-methylbu-tanoyl )-dihydrolipoamide-E, cis-4-hydroxycyclohexy-lacetic acid, phenylbutyrylglutamine, 3-butyn-1-al, 3-hydroxytetradecanedioic acid, dihydrolipoamide and pyruvic acid, etc. were characterized. Conclusions The results indicate that Shen-Mai decoction can pro-tect the body from myocardial injury by regulating pu-rine metabolism, some acid metabolism, fat metabo-lism and energy metabolism, etc. The study expounds the functioning mechanism for Shen-Mai decoction ’ s medical effect in the body and provides theoretical grounds for the rationality of the two medical herbs ’ compatibility and their combination in clinical treat-ment of diseases.
