Protective effect of naringin on vascular endothelial injury induced by high glucose associated with reduced expression of CX3 CL1 and antioxidant
10.3969/j.issn.1001-1978.2016.11.024
- VernacularTitle:柚皮苷对高糖引起血管内皮损伤的保护作用与CX3 CL1及抗氧化有关
- Author:
Yurong XU
;
Xuan SHENG
;
Jingjing GUO
;
Huaide JIANG
;
Shangdong LIANG
;
Guilin LI
- Publication Type:Journal Article
- Keywords:
diabetes;
naringin;
CX3 CL1;
vascular endothelial cells;
nitric oxide;
reactive oxygen species
- From:
Chinese Pharmacological Bulletin
2016;32(11):1608-1612,1613
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effect of the naringin on chemotactic factor CX3 CL1 of human umbilical vein endothelial cells ( HUVEC) induced by high glucose. Methods The effect of different concen-trations of naringin on HUVEC cell viability was deter-mined by MTS. HUVECs were divided into 4 groups:① control group, ② high glucose group, ③ naringin group and④high glucose treated with naringin group. After treatment for 5 days, the concentration of nitric oxide ( NO ) in the culture medium was measured by nitrate reductase; intracellular reactive oxygen species ( ROS) was analyzed with fluorescence probe; the ex-pressions of CX3 CL1 mRNA and protein were deter-mined by the reverse transcription PCR ( RT-PCR ) and Western blot ( WB ) . Results NO content in the culture medium of high glucose group was markedly de-creased, which could be increased by naringin. Com-pared with the control group, intracellular ROS in the high glucose group was drastically elevated, but narin-gin decreased the elevated ROS induced by high glu-cose. The results of RT-PCR and WB showed that nar-ingin could downregulate the increased expressions of CX3CL1 mRNA and protein induced by high glucose. Conclusion Naringin has protective effect on the in-jury of the HUVEC induced by high glucose, which is associated with reducing the expression of CX3 CL1 and the antioxidative and anti-inflammatory action.
