Optimization of the Extraction Technology for Oligosaccharides of Morinda officinalis by Response Surface Methodology
10.6039/j.issn.1001-0408.2015.34.31
- VernacularTitle:响应面法优化巴戟天低聚糖提取工艺
- Author:
Xin YANG
;
Jianping SONG
;
Yezhi GUAN
;
Qi WANG
;
Li LIN
;
Weidong LIN
;
Shanxi GU
- Publication Type:Journal Article
- Keywords:
Morinda officinalis How;
Oligosaccharides;
Extraction technology;
Response surface methodology;
Optimization
- From:
China Pharmacy
2015;(34):4847-4849,4850
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of oligosaccharides of Morinda officinalis. METHODS:The liq-uid/solid ratio,extraction time and temperature were chosen as factors,and the yield of oligosaccharides was estimated as index. On the basis of single-factor experiments,using a 3-factor,3-level Box-behnken central composite experimental design,two-poly-nomial regression equation of extraction rate of oligosaccharide was established,and it was analyzed by response surface methodolo-gy to obtain the optimum extraction conditions,and the verification test was conducted. RESULTS:The optimum extraction condi-tions were as follows as material/liquid ratio of 23∶1(ml/g),extraction time of 1.7 h,and extraction temperature of 93℃,extract-ing for twice. Under these conditions, the estimated and observed average values of extraction rate of oligosaccharides were 10.37% and 10.29%(RSD=0.20%,n=3),respectively. The deviation value was 0.06%. CONCLUSIONS:The response surface methodology can be used to optimize the extraction process of oligosaccharides of M. officinalis.
