Study on the Quality Standard of Jinbo Capsule
10.6039/j.issn.1001-0408.2015.30.24
- VernacularTitle:金柏胶囊的质量标准研究Δ
- Author:
Wan LEI
;
Yuxia KANG
;
Ying WANG
;
Feiyan GUO
;
Xiaojuan WANG
- Publication Type:Journal Article
- Keywords:
Jinbo capsule;
Berberine hydrochloride;
TLC;
HPLC
- From:
China Pharmacy
2015;(30):4245-4246,4247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To provide reference for the establishment of quality standard for Jinbo capsule. METHODS:TLC was conducted for the qualitative identification of Phellodendron chinense and Melia toosendan in preparation;HPLC was used for the content determination of berberine hydrochloride in preparation;the column was Diamonsil C18 with mobile phase of acetoni-trile-0.1% phosphoric acid (50∶50,V/V) at flow rate of 1 ml/min,detection wavelength was 265 nm,column temperature was 25 ℃ and volume injection was 10 μl. RESULTS:TLC spots of P. chinense and M. toosendan in preparation were clear and well-separated. The linear range of berberine hydrochloride was 0.044-0.154 mg/ml(r=0.999 9);RSDs of precision,reproducibili-ty and stability tests were no more than 2.0%,recovery was 98.36%-102.15%(RSD=1.11%,n=9). CONCLUSIONS:The meth-od is simple,accurate and reproducible,and can be used for the quality control of Jinbo capsule.
