Study on the Quality Standard of Fresh Gastrodia elata Lyophilized Powder

VernacularTitle:鲜天麻冻干粉的质量标准研究
Author: Aiping ZUO ; Chuanfang WANG ; Mei PAN ; Jingwen TANG ; Liyan ZHANG ; Hao WU
Publication Type:Journal Article
Keywords: Fresh Gastrodia elata lyophilized powder; Quality standard; TLC; HPLC
From: China Pharmacy 2015;(30):4250-4251,4252
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To provide reference for the establishment of quality standard for Fresh Gastrodia elata lyophilized powder. METHODS:TLC was conducted for the qualitative identification of G. elata in preparation,and HPLC was conducted for the content determination of gastrodin in preparation. The column was Diamonsil C18 with mobile phase of acetonitrile-0.05% phos-phoric acid(3∶97,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 25 ℃ and vol-ume injection was 10 μl. RESULTS:TLC pots of Fresh G. elata lyophilized powder were clear and well-separated. The linear of gastrodin was 2.9-14.5 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.00%;recov-ery was 98.07%-102.70%(RSD=1.74%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible,and suitable for the quality control of Fresh G. elata lyophilized powder.