Contents Determination of Rosin Acid in Rheumatoid Arthritis Tablet by HPLC-MS/MS

VernacularTitle:HPLC-MS/MS法测定风湿关节炎片中松香酸的含量
Author: Jianlong YANG ; Yanli QU ; Tongtong XU ; Xiao XING ; Yanfei ZHANG ; Jiyu GONG
Publication Type:Journal Article
Keywords: Rheumatoid arthritis tablet; HPLC-MS/MS; Rosin acid
From: China Pharmacy 2015;(30):4299-4300,4301
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the content determination of rosin acid in Rheumatoid arthritis tablet. METH-ODS:HPLC was performed on the column of ZORBAX SB-C18 with mobile phase of acetonitrile-0.1% formic acid(82∶18,V/V) at flow rate of 1.0 ml/min ,detection wavelength was 241 nm ,column temperature was 30 ℃ and volume injection was 10 μl. MS/MS column was ZORBAX SB-C18 with mobile phase of acetonitrile-0.1% formic acid(80∶20,V/V)at flow rate of 0.2 ml/min;column temperature was 30 ℃;volume injection was 0.5 μl. Ionization mode was ESI+,atomization gas pressure was 25 psi,gas flow as 8.0 L/min,capillary voltage was 4 000 V,capillary outlet voltage was 120 V,precursor ion was 303 m/z,scan range was 50-500 m/z and the gas temperature was 350 ℃. RESULTS:The linear range of rosin acid was 2.5-100.0 μg/ml. RSDs of preci-sion,stability and reproducibility tests were lower than 2.0%,recoveries was 96.75%-98.11%(RSD=0.53%,n=6). CONCLU-SIONS:The method is simple,accurate and reproducible,and suitable for the content determination of rosin acid in Rheumatoid arthritis tablet.