Simultaneous Determination of Spironolactone and Erythromycin in Compound Spironolactone Gel by HPLC
10.6039/j.issn.1001-0408.2015.30.42
- VernacularTitle:HPLC法同时测定复方螺内酯凝胶中螺内酯和红霉素的含量
- Author:
Qizhong FAN
;
Xinxin LI
;
Mingxiang BO
;
Limin HU
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound spironolactone gel;
Spironolactone;
Erythromycin;
Content determination
- From:
China Pharmacy
2015;(30):4295-4296,4297
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of spironolactone and erythromycin in Com-pound spironolactone gel. METHODS:HPLC was performed on the column of Thermo-Hypersil ODS2-C18 with mobile phase of 0.1 mol/L ammonium dihydrogen phosphate solution (pH was adjusted to 7.0 by triethylamine)-acetonitrile (60∶40,V/V) at flow rate of 1.0 ml/min,detection wavelength was 215 nm and 238 nm,column temperature was 30℃,and injection volume was 5 μl. RESULTS:The linear range was 0.251 6-5.032 μg/ml for spironolactone and 0.577 2-11.544 μg/ml(r=0.999 9) for erythromycin (r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.83%;average recoveries were 97.8%(RSD=0.74%,n=9)and 96.7%(RSD=2.60%,n=9),respectively. CONCLUSIONS:The method is simple,reproducible,ac-curate and reliable,and can be used for the quality control of Compound spironolactone gel.
