Effects of Shenfu Qiangxin Pills on the Expression of LC3b and Bax in Myocardial Cells of Rats with Car-diorenal Syndrome
10.6039/j.issn.1001-0408.2016.19.04
- VernacularTitle:参附强心丸对心肾综合征模型大鼠心肌细胞中LC3b、Bax表达的影响Δ
- Author:
Xu LI
;
Zi WANG
;
Di HAO
;
Lei WANG
- Publication Type:Journal Article
- Keywords:
Shenfu qiangxin pills;
Cardiorenal syndrome;
Autophagy-associated protein;
Aldosterone;
Rats;
Pro apoptotic gene
- From:
China Pharmacy
2016;27(19):2602-2604,2605
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Shenfu qiangxin(SFQX)pills on the expression of autophagy-associated pro-tein LC3b and pro apoptotic gene Bax in myocardial cells of rats with cardiorenal syndrome (CRS). METHODS:Rats were ran-domly divided into sham operation group(water),model group(water),positive control group(Captopril tablets 2.3 mg/kg)and SFQX pills high-dose,medium-dose and low-dose groups [13.2,6.6,3.3 g(crude drug)/kg],with 10 rats in each group. CRS mod-el was induced in those groups by abdominal-aortae-constriction+acute renal ischemia reperfusion injury except for sham operation group;and they were given relevant medicine intragastrically 8 week after operation,once a day,for consecutive 4 weeks. Plasma contents of Cr and ALD,the protein expression of LC3b and Bax in myocardial tissue of rats were detected 24 h after last medica-tion;ventricular index was calculated,and morphological change of myocardial tissue was observed. RESULTS:Compared with sham operation group,the plasma contents of Cr and ALD,ventricular index and the protein expression of LC3b in myocardial tis-sue increased significantly in model group (P<0.05 or P<0.01);and myocardial cell suffered from endochylema red deletion, myocardial cross striation disorder,intercellular space fibrosis aggravation and so on. Compared with model group,the plasma con-tents of Cr and ALD(except for positive control group)and the protein expression of LC3b and Bax in myocardial tissue decreased significantly in positive control group and SFQX pills high-dose group(P<0.05 or P<0.01);myocardial pathological change was improved;the ventricular index decreased significantly in SFQX pills low-dose and medium-dose groups (P<0.05). CONCLU-SIONS:SFQX pills can decrease the plasma contents of Cr and ALD,inhibit myocardial cell autophagy and apoptosis in CRS rats.
