Effects of Simvastatin on Oxidative Stress and Cell Apoptosis in Aged Mice with Myocardial Ischemia-re-perfusion
10.6039/j.issn.1001-0408.2016.19.11
- VernacularTitle:辛伐他汀对老年小鼠心肌缺血再灌注时氧化应激及细胞凋亡的影响
- Author:
Xiaolong LIAO
;
Shouhong WANG
;
Zhonghua WANG
;
Weixin GUO
;
Jianyi WEN
;
Tiehe QIN
- Publication Type:Journal Article
- Keywords:
Simvastatin;
Myocardial ischemia-reperfusion;
Oxidative stress;
Myocardial cell apoptosis;
Aged mice
- From:
China Pharmacy
2016;27(19):2626-2628,2629
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of simvastatin on oxidative stress and cell apoptosis in aged mice with myocardi-al ischemia-reperfusion (IR). METHODS:Aged mice were randomly divided into sham operation group (phosphate buffer solu-tion),model group(phosphate buffer solution)and simvastatin low-dose,medium-dose and high-dose groups(2.5,5 and 20 mg/kg) with 14 mice in each group. Those groups were given relevant medicine intraperitoneally before modeling for 7 d,once a day. IR model was induced in those groups except for sham operation group. The area ratio of myocardial infarction,myocardial cell apop-tosis rate,activity of myocardial tissue apoptosis gene Caspase-3,the protein expression of Bax and Bcl-2,Akt phosphorylation, serum concent of MDA and activity of SOD were all detected. RESULTS:Compared with sham operation group,the area ratio of myocardial infarction,myocardial cell apoptosis rate,Caspase-3 activity,the protein expression of Bax and MDA content were all increased in model group,while the protein expression of Bcl-2,Akt phosphorylation and SOD activity were decreased(P<0.01). Compared with model group,the area ratio of myocardial infarction,myocardial apoptosis rate,Caspase-3 activity,the protein ex-pression of Bax and MDA content were all decreased in simvastatin high-dose group,while the protein expression of Bcl-2,Akt phosphorylation and SOD activity were increased (P<0.01). There was no statistical significance in above indexes in simvastatin low-dose and medium-dose groups (P>0.05). CONCLUSIONS:Simvastatin can relieve myocardial IR injury in aged mice,and the mechanism of which may be associated with inhibiting myocardial cell apoptosis and the generation of oxidative stress.
