Preparation of Folic Acid-loaded Vincristine Nano Liposome and Its Effects on Human Liver and Lung Cancer Cells
10.6039/j.issn.1001-0408.2016.19.31
- VernacularTitle:叶酸修饰的长春新碱纳米脂质体的制备及其对人肝、肺癌细胞的作用
- Author:
Weizheng KOU
;
Xiaoyu YANG
;
Liuzhong YANG
;
Dan YANG
;
Yinghua JI
- Publication Type:Journal Article
- Keywords:
Folic acid-loaded vincristine nano liposome;
Liver-targeting;
Liver cancer HepG2 cells;
Lung cancer A549 cells
- From:
China Pharmacy
2016;27(19):2690-2692,2693
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare folic acid(FA)-loaded vincristine(VCR)nano liposome(VCR-nLip-FA)and to study its effects on human liver and lung cancer cells. METHODS:VCR-nLip-FA was prepared by ammonium sulfate gradient method,and particle size,Zeta-potential,encapsulation rate and release rate were investigated. Taking human liver cancer HepG2 cells and lung cancer A549 cells as example,uptake rate and inhibitory effect in vitro (5-80 μg/ml) were compared between VCR-nLip-FA and VCR-nLip. RESULTS:The particle size distribution,average particle size,average Zeta-potential,average encapsulation rate and 24 h accumulative release rate of VCR-nLip-FA were 98.1-159.0 nm,132.2 nm,-40.1 mV,(86.6±3.5)%(n=4)and(42.2± 2.6)%. Compared with VCR-nLip,there was no statistical significance in uptake rate of A549 cells to VCR-nLip-FA and inhibitory effect of VCR-nLip-FA on A549 cell viability (P>0.05);uptake rate of HepG2 cells to VCR-nLip-FA and inhibitory effect of VCR-nLip-FA on HepG2 cell viability increased significantly (P<0.01),in dose-dependent manner. CONCLUSIONS:Prepared VCR-nLip-FA can target anti-tumor drug to HepG2 cells efficiently,and highly inhibit the growth of HepG2 cells. But it has no higher effects on A549 cells.
