Research on the Extraction Technology of Glucosinolates from Uighur Medicine Brassica rapa L.

VernacularTitle:维药恰玛古中硫代葡萄糖苷的提取工艺研究
Author: Tao ZHANG ; Xiqiang AN ; Li HUANG ; Xu HU ; Jiangnan CHENG ; Tingting ZHAO ; Yuan MA ; Juan ZHANG
Publication Type:Journal Article
Keywords: Brassica rapa L.; Glucosinolates; Single factor test; Orthogonal test; Extraction technology; Optimization
From: China Pharmacy 2015;(25):3548-3550,3551
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To optimize the extraction technology of glucosinolates from Uighur medicine Brassica rapa L.. METHODS:Ethanol refluxing method was adopted to extract glucosinolates from Uighur medicine B. rapa L.. With the comprehen-sive score of glucosinolates and dry extract yield as the index,L9(34)orthogonal test based on the single factor test was adopted to observe the effects of ethanol volume fraction,extraction time and material-liquid ratio on the extraction technology of glucosino-lates from B. rapa L.,and verification test was conducted. RESULTS:The optimal extraction technology was to add 95% ethanol 8 times as much as the amount of the herbs,twice for 1.0 h reflux extractions. The extraction amount of glucosinolates and dry ex-tract yield were 7.36 mg/g and 25.29% at average,respectively.The comprehensive score of RSD was 0.52%(n=3). CONCLU-SIONS:The optimal extraction technology is stable and feasible and can be used for the extraction of glucosinolates from B. rapa L..