Study on in vitro Effects of TanshinoneⅡA to Reverse Multiple Drug Resistance
10.6039/j.issn.1001-0408.2015.25.11
- VernacularTitle:丹参酮ⅡA逆转多药耐药的体外效应研究
- Author:
Deng PAN
;
Kehe CHEN
- Publication Type:Journal Article
- Keywords:
TanshinoneⅡA;
Multiple drug resistance;
Lung cancer;
Breast cancer;
Cell viability
- From:
China Pharmacy
2015;(25):3488-3489,3490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study in vitro effects of tanshinoneⅡA to reverse multiple drug resistance. METHODS:MCF-7/ADM cells and A549/DDP cells were cultured with 0 [20 mg/L doxorubicin(ADM)or cisplatin(DDP),negative control],5 mg/ml tanshinone ⅡA(combined with 20 mg/L ADM or DDP)for 24 and 48 h. Then MTT method was used to determine cell viability, and polymerase chain reaction(PCR)was adopted to detect mRNA expressions of cell cycle control protein CDC25A and cell cy-clin dependent kinase (CKD2). RESULTS:Compared to the negative control,after MCF-7/ADM cells and A549/DDP cells were cultured with tanshinone ⅡA for 24 and 48 h,cell viability was weaker,also were mRNA expressions of CDC25A and CKD2. There were statistical differences(P<0.01). CONCLUSIONS:Tanshinone ⅡA combined with ADM or DDP can inhibit the viabili-ty of cell line MCF-7/ADM and cell line A549/DDP,decrease expression of CDC25A,CKD2 mRNA in cells and reverse multiple drug resistance in malignant tumors.
