Content Determination of Dihydroartemisinin in Dihydroartemisinin and Piperaquine Phosphate Tablets by HPLC
10.6039/j.issn.1001-0408.2015.24.29
- VernacularTitle:HPLC法测定双氢青蒿素哌喹片中双氢青蒿素的含量Δ
- Author:
Lin ZHOU
- Publication Type:Journal Article
- Keywords:
HPLC;
Dihydroartemisinin and piperaquine phosphate tablet;
Dihydroartemisinin;
Content determination
- From:
China Pharmacy
2015;(24):3393-3394,3395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of dihydroartemisinin in Dihydroartemisinin and piper-aquine phosphate tablets. METHODS:HPLC was performed on the column of Waters YMC-Pack ODS-AQ with mobile phase of acetonitrile-water(gradient elution)at the flow rate of 1.0 ml/min,the detection wavelength was 216 nm,column temperature was 30℃,and injection volume was 20 μl. RESULTS:The linear range of dihydroartemisinin was 0.009 650-1.930 mg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.5%;average recovery was 100.76%(RSD=0.95%,n=9). CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the content determination of dihydroartemis-inin in Dihydroartemisinin and piperaquine phosphate tablets.
