Simultaneous Determination of 5 Components in Shenshao Oral Liquid by Multi-wavelength RP-HPLC Method
10.6039/j.issn.1001-0408.2015.24.38
- VernacularTitle:多波长RP-HPLC法同时测定参芍口服液中5种成分的含量
- Author:
Lianhao FU
;
Xijie ZHANG
;
Shaozhi WANG
;
Xiaohong LIU
- Publication Type:Journal Article
- Keywords:
Multi-wavelength RP-HPLC;
Shenshao oral liquid;
Tanshinol;
Protocatechuic aldehyde;
Paeoniflorin;
Ferulic ac-id;
Salvianolic acid B
- From:
China Pharmacy
2015;(24):3423-3425,3426
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of tanshinol,protocatechuic aldehyde,paeoni-florin,ferulic acid and salvianolic acid B in Shenshao oral liquid. METHODS:RP-HPLC was performed on the column of Eclipse XDB C18 with mobile phase of 0.5%phosphoric acid-methanol-acetonitrile(gradient elution)at the flow rate of 1.0 ml/min,the de-tection wavelength was 280,230 and 320 nm,column temperature was 30 ℃ and volume was 10 μl. RESULTS:Under the chro-matographic conditions,5 kinds of components could be completely separated,the linear range of tanshinol,protocatechuic alde-hyde,paeoniflorin,ferulic acid and salvianolic acid B were respectively 24-384 μg/ml(r=0.999 9),1.25-20 μg/ml(r=0.999 9), 40.5-648 μg/ml(r=0.999 8),1.5-24 μg/ml(r=0.999 9),145-2 320 μg/ml(r=0.999 9);RSDs of precision,stability and reproduc-ibility tests were no more than 2.2%;the average recovery was respectively 100.7%(RSD=1.23%,n=9),100.0%(RSD=2.19%,n=9),99.6%(RSD=0.87%,n=9),100.3%(RSD=1.11%,n=9) and 99.3%(RSD=2.46%,n=9). CONCLUSIONS:The method is specific with good precision and reproducibility,and can be used for the content determination of 5 components in Shenshao oral liquid.
