Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection.
- Author:
Hyeong Woo LEE
1
;
Sung Ung MOON
;
Hye Sun RYU
;
Yeon Joo KIM
;
Shin Hyeong CHO
;
Gyung Tae CHUNG
;
Khin LIN
;
Byoung Kuk NA
;
Yoon KONG
;
Kyung Suk CHUNG
;
Tong Soo KIM
Author Information
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords: Plasmodium falciparum; falciparum malaria; liver stage antigen-3; recombinant protein; serodiagnosis
- MeSH: Recombinant Proteins/biosynthesis/genetics/*immunology; Plasmodium vivax/isolation & purification; Plasmodium falciparum/*immunology; Molecular Sequence Data; Malaria, Falciparum/blood/*diagnosis; Humans; Genes, Protozoan/genetics/immunology; Fluorescent Antibody Technique, Direct/methods; Escherichia coli/genetics; Enzyme-Linked Immunosorbent Assay/methods; Early Diagnosis; DNA, Protozoan/chemistry; DNA Primers/chemistry; Cloning, Molecular/methods; Base Sequence; Antigens, Protozoan/biosynthesis/chemistry/genetics/*immunology; Animals; Amino Acid Sequence
- From:The Korean Journal of Parasitology 2006;44(1):49-54
- CountryRepublic of Korea
- Language:English
- Abstract: In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
