Preparation of Curcumin Solid Lipid Nanoparticles by Microemulsion Method
10.6039/j.issn.1001-0408.2015.19.33
- VernacularTitle:微乳法制备姜黄素固体脂质纳米粒
- Author:
Nan LI
;
Xijing LI
;
Qian WANG
- Publication Type:Journal Article
- Keywords:
Curcumin;
Microemulsion method;
Solid lipid nanoparticles;
Pseudo-ternary phase diagram;
Encapsulation effi-ciency;
Orthogonal test
- From:
China Pharmacy
2015;(19):2698-2701,2702
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare the curcumin solid lipid nanoparticles. METHODS:Microemulsion method was adopted to prepare curcumin solid lipid nanoparticles (SLN) and pseudo-ternary phase diagrams was conducted to optimize the preferable three-phase microemulsion factors. The optimal conditions were determined,and then hot microemulsion was dispersed in cold wa-ter to prepare SLN. After the preliminary screening of the factors (emulsifiers,lipid materials,amount of lipids,drug-to-lipid ra-tio,cold water phase temperature and the holding temperature of microemulsion)by single-factor test,with the index of encapsula-tion efficiency,the orthogonal test was conducted to optimize the formulation,followed by the verification test. RESULTS:The best microemulsion was produced at 65 ℃ with three phases consisting of stearic acid(oil phase),polysorbate 80(emulsifier)and ethanol (co-emulsifier) and the Km ratio of 1∶4. The optimal conditions for SLN were as follows as curcumin dosage of 50 mg, stearic acid of 0.5 g,cold water phase temperature of 2 ℃,holding temperature of microemulsion of 65 ℃. The encapsulation effi-ciency was 87.73% for solid lipid nanoparticles prepared,drug-loading rate was 7.72%,particle size was(156.9±2.2)nm,poly-dispersity coefficient was 0.480,average Zeta potential was -24.8 mV(RSD<2%,n=3). CONCLUSIONS:The microemulsion method to prepare SLN is simple and feasible.
